Immunocytochemical study of gap junction-related protein, innexin 2, in Periplaneta americana (Blattodea: Blattidae)
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چکیده
Gap junctions are intercellular junction apparatus found in almost all multicellular animals and are involved in direct intercellular communication. Those of invertebrate animals consist of the innexin protein family. We developed antibodies against peptide fragments of Bombyx mori innexin 2, and examined tissues of Periplaneta americana by Western blotting, immunohistochemistry and immunoelectron microscopy. Intense immunoreactivity of innexin 2 was found in the midleg, gizzard and thoracic muscular tissues. Immunoelectron microscopy showed that immunogold particles of innexin 2 were aggregated around cell adhesion structures between muscle fibers. These results suggest that innexin 2 is expressed in the muscle cells of cockroaches and is involved in synchronization of muscle contraction.
منابع مشابه
Tachykinin stimulates α-amylase, protease and lipase activities in the midgut of the American cockroach, Periplaneta americana (Blattodea: Blattidae)
در این تحقیق به بررسی اثر تاکیکینین بر روی فعالیت آلفا آمیلاز، پروتئاز و لیپاز در معده میانی سوسری امریکایی (L.) Periplaneta americana پرداخته شد. فعالیت ایمنوهیستوشیمیایی سلول‌های معده میانی (بخش های جلویی، میانی و عقبی) که تاکیکینین ترشح می‌کردند مورد تایید قرار گرفت. چهار هفته گرسنگی، تعداد سلول هایی را که عکس العمل ایمنوهیستوشیمیایی مقابل آنتی‌بادی تاکیکینین داشتند (Tachykinin-ir cel...
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